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HelpTest Code IMM IMMUNODEFICIENCY PANEL (T, B & NK CELL SUBSETS)
Method
Flow Cytometry
CPT(s)
| Description | CPT Code |
| CD 19 | 86355 |
| CD 3 | 86359 |
| CD 4 & CD 8 | 86360 |
| CD NK | 86357 |
Specimen Information
|
Container |
Specimen |
Temperature |
Collect Vol |
Submit Vol |
Minimum Vol |
| Sodium Heparin Tube | Whole Blood | Ambient | 4 mL | 2 mL | 2 mL |
| Purple Tube (EDTA) | Whole Blood | Ambient | 4 mL | 2 mL | 2 mL |
Samples collected in sodium Heparin must be tested within 48 hours of collection. Samples collected in EDTA must be tested within 30 hours of collection.
Reference Range
Pediatric Reference Ranges
|
Age |
CD3% |
CD4% |
CD8% |
CD19% |
CD16+56% |
Absolute CD4 |
|
0-2 Mos. |
53-84% |
35-64% |
12-28% |
6-32% |
4-18% |
1600-4000 cells/uL |
|
3-5 Mos |
51-77% |
35-56% |
12-23% |
11-41% |
3-14% |
1800-4000 cells/uL |
|
6-11 Mos. |
49-76% |
31-56% |
12-24% |
14-37% |
3-15% |
1400-4300 cells/uL |
|
12-23 Mos. |
53-75% |
32-51% |
14-30% |
16-35% |
3-15% |
1300-3400 cells/uL |
|
2-5 Yrs. |
56-75% |
28-47% |
16-30% |
14-33% |
4-17% |
700-2200 cells/uL |
|
6-11 Yrs. |
60-76% |
31-47% |
18-35% |
13-17% |
3-22% |
650-1500 cells/uL |
|
12-17 Yrs. |
56-84% |
31-52% |
18-35% |
6-23% |
3-22% |
530-1300 cells/uL |
Adult Reference Ranges
|
Subset |
% |
Absolute |
|
CD3% |
62-87% |
548-2118 cells/uL |
|
CD4% |
35-63% |
329-1427 cells/uL |
|
CD8% |
10-35% |
66-750 cells/uL |
|
CD19% |
5-22% |
<488 cells/uL |
|
CD16+56% |
5-23% |
72-425 cells/uL |
Instrumentation
Beckman Coulter Navios
LOINC Code Information
| Result Code | Reporting Name | LOINC Code |
| CD3 | CD3 | 20599-7 |
| CD4 | CD4 | 32516-7 |
| CD8 | CD8 | 32518-3 |
| CD19 | CD19 | 20593-0 |
| CDNK | CD16+CD56 | 21166-4 |
| TOTCD3 | Absolute CD3 | 20598-9 |
| TOTCD4 | Absolute CD4 | 32515-9 |
| TOTCD8 | Absolute CD8 | in process |
| TOT19 | Absolute CD19 | in process |
| TOTNK | Absolute CD16+CD56 | in process |
Performing Location
University of Vermont Medical Center
Test Schedule / Analytical Time / Test Priority
Monday – Saturday / 3 days / Not available STAT
Section
Immunology
Is the UVMMC lab NY State Certified to perform this testing? Yes/No
Yes