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Test Code 5NTD 5'-Nucleotidase, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Secondary ID

622835

Useful For

Aiding diagnosis and monitoring of hepatobiliary disease and bile duct obstructions

 

Ruling out bone pathology as the source of elevated serum alkaline phosphatase

Method Name

Coupled Enzyme Kinetic Assay

Reporting Name

5'-Nucleotidase, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Clinical Information

5'-Nucleotidase (5'NT) is an enzyme that catalyzes the hydrolysis of the phosphate group from 5'-nucleotides. Increased enzyme levels in sera are associated with certain forms of liver disease, such as intra- or extra-hepatic obstruction, and particularly in cases of hepatic carcinoma and in mastectomy patients with recurrent metastases. 5'NT is specific to hepatobiliary disease, which can make it useful in conjunction with other liver enzyme activity assays, like gamma glutamyl transferase, to differentiate between an increase in alkaline phosphatase due to cholestatic liver disease rather than bone disease (Paget disease).

Reference Values

<4.6 U/L

 

For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html.

Interpretation

An elevation of 5'-nucleotidase (5'NT) activity may be seen in all forms of liver disease, although the highest elevations are seen in intra- or extra-hepatic biliary obstruction.

 

5'-nucelotidase may be more specific for intrahepatic cholestasis than gamma glutamyl transferase.

 

Elevation of 5'NT activity is not associated with skeletal disease. Thus, measurement of 5'NT in serum can be used to differentiate observed elevations of alkaline phosphatase due to skeletal disease vs. hepatobiliary disease.

CPT Code Information

83915

LOINC Code Information

Test ID Test Order Name Order LOINC Value
5NTD 5'-Nucleotidase, S 1690-7

 

Result ID Test Result Name Result LOINC Value
5NTD 5'-Nucleotidase, S 1690-7